Developmental competence of bovine embryos from heat-stressed ova
نویسندگان
چکیده
منابع مشابه
P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes
Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...
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The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -35 degrees C or -80 degrees C in the presence of 5% DMSO were used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstructed from the somatic cells, 78% to 81% s...
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The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different developmental competence. Four oocyte populations were collected, separately either from small (2-5 mm) or medium (6-10 mm) follicles, in the growth/stagnation (G/S) or dominance/regression (D/R) stage of the first follicular wave. They were matured, fertilized and cultu...
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Many efforts have been made to develop effective culture conditions for the production of bovine blastocysts. Growth hormone (GH) and glucose are known to affect in vitro embryo development. To improve in vitro culture conditions, the culture medium containing fetal calf serum (FCS) or bovine serum albumin (BSA) was supplemented with GH at various periods of development, and the effects of GH o...
متن کاملInvolvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation.
Various pathological stimuli such as radiation, environmental toxicants, oxidative stress, and heat shock can initiate apoptosis in mammalian oocytes. Experiments were performed to examine whether apoptosis mediated by group II caspases is the cause for disruption of oocyte function by heat shock applied during maturation in cattle. Bovine cumulus-oocyte complexes (COCs) were cultured at 38.5, ...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2009
ISSN: 0022-0302
DOI: 10.3168/jds.2008-1495